This course will focus on the structure, function, design and engineering of biomacromolecules and their use in modern biotechnologies. After a brief review of protein and nucleic acid chemistry and structure, we will delve into rational, evolutionary, and computational methods for the design and engineering of these biomolecules. Then we will review applications in the primary literature including: protein and RNA-based switches and sensors, unnatural amino acids and nucleotides, enzyme engineering, integration of these parts via synthetic biology, and metabolic engineering.

This course covers major diseases (cancer, diabetes, heart disease, infectious diseases), the physical changes that inflict morbidity and mortality, the design constraints for treatment, and emerging technologies that take into account these physical hurdles. Taking the perspective of the design constraints on the system (that is, the mass transport and biophysical limitations of the human body), we will survey recent innovations from the fields of drug delivery, gene therapy, tissue engineering, and nanotechnology.

An integrated, mathematically and computationally sophisticated introduction to physics and chemistry, drawing on examples from biological systems. This year long, four course sequence is a multidisciplinary course taught across multiple departments with the following faculty: G. Scholes (CHM), T. Gregor, J. Shaevitz (PHY); Britt Adamson, John Storey, M. Wuhr (MOL); J. Gadd-Reum, H. McNamara, S. Ryabichko, B. Zhang (LSI). Five hours of lecture, one three-hour experimental lab, one three-hour computational lab.

An integrated, mathematically and computationally sophisticated introduction to physics and chemistry, drawing on examples from biological systems. This year long, four course sequence is a multidisciplinary course taught across multiple departments with the following faculty: G. Scholes (CHM), T. Gregor, J. Shaevitz (PHY); Britt Adamson, John Storey, M. Wuhr (MOL); J. Gadd-Reum, H. McNamara, S. Ryabichko, B. Zhang (LSI). Five hours of lecture, one three-hour experimental lab, one three-hour computational lab.

This course focuses on computational methods in cryo-EM, including three-dimensional ab-initio modelling, structure refinement, resolving structural variability of heterogeneous populations, particle picking, model validation, and resolution determination. Special emphasis is given to methods that play a significant role in many other data science applications. These comprise of key elements of statistical inference, image processing, optimization, and dimensionality reduction. The software packages RELION and ASPIRE are routinely used for class demonstration on both simulated and publicly available experimental datasets.

Important concepts and elements of molecular biology, biochemistry, genetics, and cell biology, are examined in an experimental context. This course fulfills the basic biology requirement for students majoring in the biological sciences and satisfies the basic biology requirement for entrance into medical school and most other health professions schools.

The current environment in the US for the use and abuse of foods and drugs will be examined from a scientific fact-based perspective. Historical, economic, marketing, political, and public health drivers will be considered. Specific topics include government dietary recommendations (food politics), dietary supplements (from Vitamins to herbal extracts), pharmacology and ethical drug development (sulfa drugs, NSAIDS, etc), addiction and substance abuse (alcohol, nicotine, stimulants, opioids, etc), Alzheimer's disease and the problem of long-term care in an aging population, and Psychedelic drug use and abuse (psilocybin, mescaline, LSD, etc).

MOL320 is a spring semester course offered to sophomores intending to concentrate in MOL and plan to study abroad, or have taken/are concurrently taking MOL348 and want an early introduction to research methods & laboratory experience. The purpose of MOL320 is to prepare you to be a contributing member of a research lab and to foster creative, critical thinking and effective communication skills. While completing original research, you will employ techniques used by both molecular biologists and developmental geneticists. You will explore scientific literature to understand prior research and will generate a final research paper on your work.

A broad survey of the field of immunology and the mammalian immune system. The cellular and molecular basis of innate and acquired immunity will be discussed in detail. The course will provide frequent examples drawn from human biology in health and disease.

Basic principles of genetics illustrated with examples from prokaryote and eukaryote organisms. Classical genetic techniques as well as molecular and genomic approaches will be discussed. The evolving concept of the gene, of genetic interactions and gene networks, as well as chromosome mechanics will be the focus of the course. Selected topics will include gene regulation, cancer genetics, the human biome, imprinting, and stem cells.

This course focuses on the fundamental biochemical principles that underlie cellular function. An emphasis will be placed on protein structure, function, and regulation. Additional topics covered will include metabolism and catalysis, and cutting-edge methodologies for studying macromolecules in health and disease systems.

The course will investigate the roles that gene regulation, cell-cell communication, cell adhesion, cell motility, signal transduction and intracellular trafficking play in the commitment, differentiation and assembly of cells into specialized tissues. The mechanisms that underlie development of multicellular organisms, from C. elegans to humans, will be examined using biochemical, genetic and cell biological approaches. In-class problem solving, group work, and active learning approaches will be used to emphasize key concepts and analyze experimental data.

The ultimate goal of every species is the successful contribution of an individual's genes to the next generation, leading to the evolution of diverse strategies to maximize reproductive success. First, we discuss various reproductive tactics employed in the animal kingdom, examining topics such as asexual and sexual reproduction, extent of parental investment, and maximizing reproductive lifespan. We then focus on human reproductive biology, highlighting age and environment-induced fertility issues, as well as cutting edge research into fertility treatments and assisted reproductive technologies employed in clinics which combat these issues.

We will explore the molecular events leading to the onset and progression of human cancer. We will review the central genetic and biochemical elements that make up the cell cycle, followed by a survey of the signal transduction pathways and checkpoints that regulate it. We will discuss oncogenes, tumor suppressor and mutator genes that act in these pathways and review the role of viral oncogenes and their action on cells. We will investigate the role of cancer stem cells and the interaction between tumor and the host environment. We will explore specific clinical case studies in light of the molecular events underlying different cancers.

This course will consider the principles, development, outcomes and future directions of therapeutic applications of biotechnology, with particular emphasis on the interplay between basic research and clinical experience. Topics to be discussed include production of hormones and other protein drugs, nucleic acid drugs and vaccines, gene therapy and gene editing, and molecular diagnostics. Reading will largely be from the primary literature.

Many organisms are agents of disease in humans, but few can cause a pandemic. This course will survey where pandemic pathogens come from, how they replicate and cause disease, and what technologies have been invented to combat them or predict where they may emerge next.

The cell biology of tissues is discussed covering the molecules and fundamental concepts in cell communication, adhesion, shape, division, and differentiation. How cells become different from one another in a developing organism is explored, focusing on important concepts and developmental strategies using model systems. Primary literature is used to introduce seminal work, classic and modern experimental approaches, and outstanding questions in cell and developmental biology. Students are expected to learn to read critically, think beyond the reading, and participate in presenting and discussing the materials.

Modern biology research increasingly relies on quantitative tools to make precise measurements of cell state. This course provides an introduction to the experimental techniques and computational methods that enable the quantitative study of biological systems. We start with an intro to programming using Python and we employ the learned skills to analyze proteomics and sequencing data for studying gene networks within and across species, modeling biochemical reactions to study the dynamics of gene and protein networks, and extracting information about the spatial organization of biological systems using fluorescence imaging.

We explore the molecular events leading to the onset and progression of human cancer. We review the central genetic and biochemical elements that make up the cell cycle, followed by a survey of the signal transduction pathways and checkpoints that regulate it. We discuss oncogenes, tumor suppressor and mutator genes that act in these pathways and review the role of viral oncogenes and their action on cells. We investigate the role of cancer stem cells and the interaction between tumor and the host environment. We explore specific clinical case studies in light of the molecular events underlying different cancers.

Students perform research in the laboratories of potential faculty advisors.

Satisfies the NIH mandate for training in the ethical practice of science. The course is discussion-based, and uses readings, videos, case studies and guest participants to examine basic ethical and regulatory requirements for the responsible conduct of research. Topics include: the nature of - and response to - research misconduct; collaborative research; protection of human and animal subjects; conflicts of interest and commitment; authorship, publication and peer review; mentorship; societal impacts of scientific research; diversity and inclusion in scientific research; and contemporary ethical issues in biomedical research.

Introduction to a mathematical description of how networks of neurons can represent information and compute with it. Course will survey computational modeling and data analysis methods for neuroscience. Example topics are short-term memory and decision-making, population coding, modeling behavioral and neural data, and reinforcement learning. Classes will be a mix of lectures from the professor, and presentations of research papers by the students. Two 90 minute lectures, one laboratory. Lectures in common between NEU 437/NEU 537.

A survey of modern neuroscience that covers experimental and theoretical approaches to understanding how the brain works. This semester builds on 501, focusing on how the circuits and systems of the brain give rise to cognition. The course covers the neural mechanisms responsible for vision, long-term memory, sleep, motor control, habits, decision making, attention, working memory, and cognitive control. How these functions are disrupted in neurodegenerative and neuropsychiatric disorders are also covered. This is the second term of a double-credit core lecture course required of all Neuroscience Ph.D. students.

This lab course introduces students to the variety of experimental and computational techniques and concepts used in modern cognitive neuroscience. Topics include functional magnetic resonance imaging, scalp electrophysiological recording, and computational modeling. In-lab lectures provide students with the background necessary to understand the scientific content of the labs, but the emphasis is on the labs themselves, including student-designed experiments using these techniques. This is the second term of a double-credit core lab course required of all Neuroscience Ph.D. students.

The course will teach core principles of the life sciences through a set of key questions that biologists have sought to answer over the past 200 years. We will read historic scientific publications, discussing the basic biology at stake as well as what enabled each scientist to see something new. In addition, we will schedule several hands-on sessions with relevant materials. By situating key findings in their place and time we show how science is an inquiry-based, concrete, and ongoing activity, rather than codified and unchanging knowledge. Topics include cell theory, evolution, experimental embryology, genetics, and molecular development.