Light Microscopy and Biological Imaging

The course will be a detailed overview of the practice of light microscopy as applied to scientific investigation. The emphasis of the course will be on the use of the light microscope by biological scientists, however students of other disciplines are welcome. We will cover optical microscope theory, microscope components and mechanics, and all modern optical techniques from brightfield through super-resolution and lightsheet microscopies. Instruction will consist of lectures, demonstrations, and hands-on experience using the light microscopes in the Molecular Biology Confocal Imaging Facility (CIF) and others provided by vendors.